FIGURE 3.

ERManI activity is not required for association of H2a with EDEM1. A, HEK 293 cells were cotransfected with a vector encoding for HA-tagged EDEM1 (EDEM1-HA) with or without an H2a encoding vector and with or without a pSUPER plasmid encoding for anti-ERManI shRNA (shERManI). 24 h post-transfection, cells were lysed and processed, and results were quantitated and normalized as in Fig. 2A. B, in parallel with A, HEK 293 cells were transfected with the plasmid encoding anti-ERManI shRNA used in A or with pSUPER encoding anti-LacZ shRNA (control). RNA was extracted 24 h post-transfection and used for RT-PCR with primers for ERManI mRNA (upper panel) compared with GAPDH (lower panel). IP, immunoprecipitation.