FIGURE 4.

Inhibition of endothelial cell apoptosis by recombinant APC N-linked glycan variants. A, endothelial cell apoptosis was measured by accumulation of apoptosis-specific dye (pink-purple) in staurosporine-treated EA.hy926 cells following incubation with wild type/variant APC. Untreated/staurosporine-treated EA.hy926 cells (top panels) and wild type/variant APC (1.25 nm, middle and bottom panels) are shown. Images are representative of three independent experiments. B, APC concentration-dependent reduction in endothelial cell apoptosis (●, wild type APC; ♢, APC-N248Q; △, APC-N313Q; □, APC-N329Q; 0.625–20 nm). Uptake of apoptosis-specific dye was quantified by converting digital photograph images into pixel counts using Adobe® Photoshop® software according to manufacturer's instructions. Average pixel counts calculated were based on analysis of at least three images per well.