FIGURE 1.

Ca²⁺-activated Cl⁻ currents induced by fluorescent protein-tagged mouse Ano1 constructs are identical to wild type Ano1. HEK293 cells were transiently transfected and subjected 1 day later to whole cell patch clamp recording. A, total RNA was collected from untransfected HEK293 cells and RT-PCR was performed to determine which Ano proteins are endogenously expressed. Ano5, -6, and -10 were strongly expressed, whereas Ano2 and -4 were mildly expressed. Ano1, -3, -7, -8, and -9 were not detectable. B, wild type mouse Ano1 (variant containing a and c alternative splice sequences, accession Q8BHY3.2). C, Ano1 tagged with eGFP on the C terminus. D, Ano1 tagged with mCherry on the C terminus. E, steady-state current-voltage relationships (red square, wild type; black square, mCherry-tagged Ano1; blue square, eGFP-tagged Ano1; circle, untransfected). All n = 10. N.B., in some cases, error bars were obscured by the symbols. * denotes p < 0.05 different to basal currents.