Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Nov 5;286(2):1381–1388. doi: 10.1074/jbc.M110.174847

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 2. — eGFP linked to mCherry with 5 glycines (eGFP-5G-mCherry) undergoes near-maximal FRET. A, typical confocal micrographs of eGFP-5G-mCherry (green, donor; red, acceptor) before and after photobleaching of the acceptor fluorophore. B, graph showing typical acceptor photobleaching over 10 s. C, resultant FRET image from A. D, mean FRET efficiency (%E) of unlinked eGFP and mCherry cotransfected in the same cells (n = 10) and from eGFP-5G-mCherry (n = 14). * denotes p < 0.05 different between linked and unlinked eGFP and mCherry. Scale bar represents 25 μm.