TABLE 1.
Primers used in RT-PCR to detect endogenous anoctamin expression in HEK293 cells
| Gene | Sense | Antisense |
|---|---|---|
| Ano1 | GCGTCCACATCATCAACATC | ATCCTCGTGGTAGTCCATCG |
| Ano2 | TGCCTACCACTACCGGAAAC | ACTTCTTTGCAATGCTGCCT |
| Ano3 | AAACCTGAACCACATCAGCC | TCTTCCCAAAAAGAAAGCGA |
| Ano4 | CATGGGAAGTCCTTGGAAGA | GCCATTGGTAAGCAAACGAT |
| Ano5 | ACACTTCACCAGAATTGGGC | GAAGCTGCTGCTGTTCCTCT |
| Ano6 | CAGTTTGGGTTCGTCACCTT | AGTACGGGTTTCCCTTGCTT |
| Ano7 | CTACTCCTGCCGGTTCAGAG | GTTCCTGCGTGGGTATGTCT |
| Ano8 | ACTTCGCTCTGCTCCTCAAG | CTTCATGACGTTGTTGGGTG |
| Ano9 | TGGAGATCAGCACCTGTGAG | CGAAGTTCACGATTCGGATT |
| Ano10 | GAGGTGCCCAGTTGTTGTTT | CCGAGTGTACCAGGTGTCCT |