Table II. Phenotypes of the mutant strains in the tethered cell assay using 1 mM propionate.
| Strain | Mutations | Anaerobic | Aerobic |
|---|---|---|---|
| WS8N | none | normal | normal |
| JPA117 | ΔOp1 | normal | normal |
| JPA403 | ΔOp2 | inverted | weak inverted |
| JPA404 | ΔOp1, ΔOp2 | smooth | smooth |
| JPA109 | Y1 | normal | normal |
| JPA114 | Y2 | normal | normal |
| JPA410 | Y3* | normal | stopped/adapts to addition |
| JPA421 | Y4 | no response | stopped/adapts to addition |
| JPA115 | Y1, Y2 | normal | normal |
| JPA412 | Y1, Y3* | normal | stopped/adapts to addition |
| JPA413 | Y2, Y3* | inverted/normal (see text) | stopped/adapts to addition |
| JPA414 | Y1, Y2, Y3* | stopped | stopped |
| JPA422 | Y1, Y4 | normal (delayed recovery) | stopped/adapts to addition |
| JPA423 | Y2, Y4 | no response | stopped/adapts to addition |
| JPA425 | Y3*, Y4 | inverted (delayed recovery) | weak normal |
| JPA424 | Y1, Y2, Y4 | no response | stopped |
| JPA427 | Y1, Y3*, Y4 | no response | weak normal |
| JPA428 | Y2, Y3*, Y4 | weak inverted | weak normal |
| JPA429 | Y1, Y2, Y3*, Y4 | inverted (delayed recovery) | weak normal |
| JPA415 | ΔOp2, Y1 | inverted (delayed recovery) | no response |
| JPA416 | ΔOp2, Y2 | inverted (delayed recovery) | no response |
| JPA417 | ΔOp2, Y1, Y2 | inverted (delayed recovery) | weak inverted |
| JPA411 | ΔOp1, Y3* | stopped | stopped/adapts to addition |
| JPA418 | ΔOp1, Y4 | normal | normal |
| JPA426 | ΔOp1, Y3*, Y4 | normal | normal |
| JPA432 | ΔOp2, Y1, Y2, Y4 | inverted (delayed recovery) | no response |
| JPA420 | ΔOp1, ΔOp2, Y4 | smooth | smooth |
| JPA419 | ΔOp2, Y4 | inverted (delayed recovery) | no response |
| JPA430 | ΔOp2, Y1, Y4 | inverted | weak inverted |
| JPA431 | ΔOp2, Y2, Y4 | inverted | no response |
See the text and figures for details of the different phenotypes. In general, a normal response is a stop-on-removal of propionate whereas an inverted response is a stop-on-addition. Strains described as having no response are able to stop but fail to respond to the addition and removal of propionate. Smooth strains are those that stop very rarely or not at all and do not respond to propionate. Note that Y1 refers to a mutation in cheY1, Y2 refers to a mutation in cheY2 and so on. ΔOp1, deletion of che operon 1; ΔOp2, deletion of che operon 2.