FIGURE 6.

BI-1 protein levels are increased in BAR-deficient cells. A, BI-1-expressing HeLa cells were transfected with scrambled siRNA or BAR siRNA (BAR siRNA-1 or BARsiRNA-2). After 24 h, cells were treated with 500 ng/ml of doxycycline for 24 h to induce BI-1 expression. Cell lysates were analyzed by immunoblotting with antibodies to HA epitope and BAR. α-Tubulin served as a loading control. B, quantification of A was performed by scanning densitometry. The scrambled siRNA group was adjusted to 1. Statistical significance (mean ± S.E.; n = 3) was determined by one-way analysis of variance and Tukey post test, and is denoted by asterisks (**, p < 0.01).