FIGURE 3.

Effect of CD44 silencing in cell adhesion and migration on hyaluronan of MeWo melanoma cells expressing or not the V3 isoform of versican. A, CD44 silencing causes a decrease in cell adhesion onto HA in MeWo LXSN and MeWo LV3SN cells. 96-well plates were coated with 5 mg/ml HA or PBS as a negative control. Cells were plated at a density of 4 × 10⁴ cells per well and allowed to attach to the plate for 16 h. Wells were stained with a 0.1% crystal violet solution for 20 min. The extent of adhesion was determined by treating cells with 0.1 m HCl and measuring the absorbance at 620 nm. A representative experiment out of four performed is shown. Small letters indicate p < 0.05 (a: versus MeWo LXSN; c: versus MeWo LV3SN). B, CD44 silencing causes a decrease in cell migration through HA in MeWo LXSN and MeWo LV3SN cells. Transwell membranes were coated with hyaluronan (5 mg/ml), cells were allowed to migrate overnight at 37 °C, and the lower side of the membranes was stained with crystal violet. A representative experiment out of three performed is shown. Small letters indicate p < 0.05 (a: versus MeWo LXSN). Values are mean ± S.D.