FIGURE 7.

Effect of versican V3 overexpression on ErbB heterodimers/CD44 interaction. A, versican V3 overexpression impairs EGFR and ErbB2-CD44 interaction in MeWo melanoma cells. Cell lysates obtained from LXSN and LV3SN MeWo melanoma cells treated with 25 ng/ml EGF for 2 min were immunoprecipitated (IP) with anti-EGFR or anti-ErbB2 or anti-CD44 followed by immunoblotting. B, ErbB/CD44 interaction is not impaired in LV3SN cells in the absence of EGF. Cell lysates obtained from LXSN and LV3SN MeWo melanoma cells were immunoprecipitated (IP) with anti-ErbB2 followed by immunoblotting with anti-CD44 or reblotting with anti-ErbB2. C, ErbB/CD44 interaction is also impaired by V3 in hyaluronidase-treated cells. Cells were incubated with 2 units/ml hyaluronidase for 2 h at 37 °C before treatment with 25 ng/ml EGF for 2 min. Cell lysates obtained from LXSN and LV3SN MeWo melanoma cells were immunoprecipitated (IP) with anti-ErbB2 followed by immunoblotting with anti-CD44 or reblotting with anti-ErbB2. PE: protein extracts.