FIGURE 5.

Genetic changes in PEX1-expressing hearts. A, histologic sections stained with either the PEX1 antibody or ANF antibody and counterstained with methyl green. Note the increased nuclear distribution of PEX1 and the concomitant up-regulation of ANF in the Tamoxifen-treated transgenics versus Tamoxifen-treated controls. B, -fold change in transcript levels relative to controls for PEX1, ANF, BNP, ACTA1, α-MHC, β-MHC, GATA4, SERCA2, NCX1, and COL1A1 in hearts of transgenic mice after 1 and 2 weeks of Tamoxifen treatment (ratio of transgenic (TG) to controls (Ctrl)). The controls of 1 and 2 weeks of Tamoxifen treatment were set at 1.The data are means ± S.E. (error bars; n = 4–5 for each group). S16 was used as the normalizer gene. *, p < 0.05 versus control (1 week); #, p < 0.05 versus control (2 weeks).