TABLE 2.
Thermodynamic parameters for interaction of JUNV ZBD with Zn2+ and acetyl-SCT determined by ITC at 25 °C
Ligand | Stoichiometry (N) | Dissociation constant (Kd) | Enthalpy (−ΔH) |
---|---|---|---|
Zn2+ | 1.81 ± 0.05a | 19.2 ± 0.4 nma | 21 ± 1 kcal mol−1a |
Acetyl-SCT | 0.86 ± 0.04a | 23.5 ± 0.3 μma | 8.7 ± 0.3 kcal mol−1a |
a The values represent averages and standard deviations of duplicate experiments.