FIGURE 6.

STAT3 knockdown attenuates increases in post-translationally modified tubulin stimulated by IL-6-type cytokines. A, cultured cardiac myocytes were transiently transfected with STAT3-targeting siRNAs (STAT3 siRNA t1, t2, or t3), a non-targeting negative control siRNA (Con siRNA) or mock-transfected without oligoribonucleotides. At 48 h post-transfection, protein lysates were prepared and blotted for STAT3, ERK expression, phosphorylated ERK, and GAPDH as a loading control. B, cardiac myocytes were transfected with STAT3-targeting siRNA or non-targeting control siRNA before stimulation with LIF (10 ng/ml, 24 h) or OSM (10 ng/ml, 24 h). Glu-, acetyl-tubulin, and α-tubulin levels were then determined by immunoblotting. GAPH was also blotted as a control for protein loading.