FIGURE 6.

Mapping of the GASP-1 binding domain in D₂ and D₃ receptor cytoplasmic tails. A, alignments of the cytoplasmic tails of the D₂-like receptors. The amino acids that were mutated are indicated in red. B, recombinant GASP-1 produced by in vitro translation was incubated with GST alone or GST fusion protein containing the cytoplasmic tail of either D₁, D₂, D₃, D₄, or D₅ or their corresponding mutants D1-Tr, D2NR, D3NR, or D4KL. Radiolabeled GASP-1 eluted from GST resin was detected (upper panel). The lower panel shows the Coomassie stain of the input protein. Shown are representative images from at least three independent experiments.