Fig. 6. Src activates αDgk in vitro. COS cells transfected with either vector alone, myc-αDgk, Src or SrcK^– were homogenized in the absence of detergent. Cell extracts were mixed as indicated in the presence of 1 mM ATP for 15 min, and were analyzed for αDgk activity (upper panel), for myc-αDgk protein by western blotting with anti-αDgk antibodies (central panel) and for Src protein by western blotting with anti-avian Src antibodies (lower panel).