SNAIL protein expression is increased by silencing LOXL2 in ATDC5 cells. Cell layers of differentiating non target and LOXL2 shRNA transduced ATDC5 cells were isolated at intervals and subjected to Western blotting for SNAIL. Expression of β-actin served as the internal control. A, representative Western blot; B, densitometric analysis of SNAIL/β-actin. The values shown are the average of three independent samples (*, p < 0.05; ***, p < 0.0005, two way ANOVA).