FIGURE 5.

LOXL2 and LOX protein levels in differentiating ATDC5 cell layers. ATDC5 cells were grown, and protein was extracted from cell layers at different time points, and equal protein samples subjected to Western blotting and, respectively, probed for (A) LOX, and (C) LOXL2; and after stripping for β-actin for normalization. LOX was expressed more in early phases of differentiation. Representative Western blots of three independent experiments are shown. Quantitative densitometry normalized to β-actin is shown for LOX (B), and for LOXL2 (D). (n = 3; **, p < 0.01; ***, p < 0.001; two way ANOVA).