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LOXL2 protein expression is inhibited by LOXL2 shRNA in differentiating ATDC5 cells. Cell layers were isolated and subjected to Western blotting using a LOXL2 antibody (A); NT, non target virus; T, LOXL2 shRNA virus. Expression of β-actin was measured for normalization. A representative blot of three independent experiments is shown. Data in B are combined from all three experiments (**, p < 0.005; ***, p < 0.0005, two way ANOVA).
