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. 2010 Nov 11;286(2):909–918. doi: 10.1074/jbc.M110.155622

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Chondrocyte differentiation markers gene expression in ATDC5 cells infected with LOXL2 shRNA lentivirus. A lentivirus knock down of LOXL2 was introduced in differentiating ATDC5 cells. RNA was extracted at day 0, 7, 14, 21, and 28, and then subjected to qPCR for expression of differentiation markers (A) SOX9, (B) type II collagen, (C) type X collagen, (D) aggrecan. All differentiation markers were significantly decreased in the ATDC5 cell line transduced with virus when compared with the non target control virus. The values shown are the average of two independent experiments done with three independent samples each (*, p < 0.05; **, p < 0.005; ***, p < 0.0005, two way ANOVA).