FIGURE 8.

Cartilage-like extracellular matrix accumulation is inhibited by LOXL2 shRNA. Top, LOXL2 and non-target lentivirus transduced ATDC5 cells were cultured, and at intervals fixed and stained with Alcian Blue as described under “Experimental Procedures.” Representative images are shown. Lower left, all stained nodules in each well were counted under a low power microscope. The values shown are the average of three independent samples (*, p < 0.05; **, p < 0.005; ***, p < 0.0005, two way ANOVA) and demonstrate that LOXL2 shRNA blocks nodule formation. Lower right, higher power image of nodules comparing the Non Target cells to the LOXL2 shRNA cells on day 35 showing mineralization nodules only in the Non Target panel. The scale bar is 0.1 mm long.