FIGURE 9.

Mineralization of ATDC5 cells is blocked by LOXL2 shRNA. A, LOXL2 and non-target lentivirus transduced ATDC5 cells were cultured, and at intervals fixed with 10% buffered formalin and stained with Alizarin Red. Representative images are shown. B, all mineralization nodules in each well were counted under a low power microscope. The values shown are the average of three independent samples (*, p < 0.05; **, p < 0.005; ***, p < 0.0005, two way ANOVA) and demonstrate that LOXL2 shRNA blocks nodule formation. C, a higher power image of nodules comparing the Non Target cells to the LOXL2 shRNA cells on day 35 showing mineralization nodules only in the Non Target panel. The scale bar is 0.1 mm long.