Fig. 1. Inactivation phenotypes of CBK1 and ACE2. Fresh overnight cultures of wild-type, Δcbk1 and Δace2 haploid strains in rich complete medium were stained with Calcofluor white (see Materials and methods). Inactivation of CBK1 and ACE2 leads to formation of large aggregates, which cannot be separated using a micro-manipulator. (A–C) The wild-type strain AW303-2b; (D–F) the Δcbk1 strain WR03-5d; (G–I) the Δace2 strain WR27-1a. (A), (D) and (G) Normaski, (B), (C), (E), (F), (H) and (I) Calcofluor white staining.