Fig. 8. Cell cycle-dependent localization of Cbk1p. Representative individual haploid cells expressing the Cbk1p–GFP fusion, taken from the experiment presented in Figure 7 have been selected to show the localization of Cbk1p at different stages of the cell cycle. During G₁ the protein is seen in a punctate pattern around plasma membrane as well as in the cytoplasm. The protein concentrates at the emerging bud site, then at the tip of the bud, dispersing around the bud as it grows. In cells with a large bud the majority of the protein forms a ring around the bud neck and just before cell separation, a plate or ring is present in both the mother and daughter cell, either side of the cell junction.