Table 1.
Relationship between germ cell death and Salmonella-mediated killing
| Condition | Germ cell corpses* | n† | TD50‡ |
|---|---|---|---|
| N2 + E. coli OP50 | 29 ± 1.8 | 20 | 16.8 ± 0.3 |
| N2 + S. typhimurium SL1344 | 91 ± 2.5 | 20 | 13.5 ± 1.5 |
| ced-3(n717) + E. coli OP50 | 0.0 | 20 | 18.8 ± 0.9 |
| ced-3(n717) + S. typhimurium SL1344 | 0.2 ± 0.4 | 20 | 5.8 ± 0.3 |
| ced-4(n1162) + E. coli OP50 | 0.0 | 15 | 13.5 ± 1.3 |
| ced-4(n1162) + S. typhimurium SL1344 | 0.0 | 19 | 6.9 ± 0.3 |
| ced-9(n1950) + E. coli OP50 | 25 ± 1.4 | 20 | 16.1 ± 0.2 |
| ced-9(n1950) + S. typhimurium SL1344 | 29 ± 1.8 | 20 | 5.6 ± 0.2 |
| egl-1(n1084n3082) + E. coli OP50 | 28 ± 0.9 | 20 | 16.9 ± 1.9 |
| egl-1(n1084n3082) + S. typhimurium SL1344 | 36 ± 1.4 | 20 | 7.5 ± 1.1 |
| N2 + S. typhimurium SL14028 (wild type) | 75 ± 2.3 | 20 | 13.0 ± 1.0 |
| N2 + S. typhimurium LH954 (phoP phoQ) | 31 ± 1.7 | 20 | 16.5 ± 0.5 |
| N2 + P. aeruginosa PA14 | 30 ± 0.4 | 15 | 3.4 ± 0.6 |
*
The germ cell corpses were scored 3 h after the infection of young adult animals by using the vital dye Syto12 as described in Materials and Methods. The number of germ corpses per gonad arm was scored only in equally stained animals.
†
n, number of animals counted.
‡
TD50s were calculated as described in Materials and Methods and data (mean ± SD) were from duplicates.