Figure 3.

pH and ionic strength conditions for optimal lysozyme activity of purified cv-lysozyme 3. Optimal pH and ionic strength were determined by measuring cv-lysozyme 3 degrading activity against a Micrococcus lysodeikticus cell wall suspended in 120 buffers covering a pH range of 3.5-10.5 and ionic strength range of I = 0.005-0.260 at 25°C (A). The conditions were compared with that of cv-lysozyme 1 [56] and cv-lysozyme 2 [57] by superimposing the optimal ionic and pH conditions of all the 3 C. virginica lysozymes; L1, L2, and L3 indicate the optimal activity conditions for cv-lysozyme 1, cv-lysozyme 2, and cv-lysozyme 3 respectively (B). Shown were percent maximum activities of each lysozyme.