Table 1.
Properties of eastern oyster lysozymes.
| Characteristic | cv-lysozyme-3 | cv-lysozyme-1* | cv-lysozyme-2** |
|---|---|---|---|
| Lysozyme activity | |||
| Optimal pH | 7.5-8.5 | 5.5-6.5 | 5.5-6.5 |
| Optimal ionic strength (I) | 0.005-0.01 | 0.18-0.20 | 0.005-0.01 |
| Antibacterial activity (MIC) | |||
| E. coli | 12.5 μg/mL | 1.56 μg/mL | 50 μg/mL |
| V. vulnificus | ND*** | 12.5 μg/mL | 100 μg/mL |
| Molecular properties | |||
| MW (Dalton)b | 17782.3 | 17861.0 | 12984.6 |
| Theoretical pI | 6.84 (7.12) | 8.95 | 6.33 |
| Amino acid sequence | |||
| Total amino acid number | 168 | 164 | 117 |
| Arginine residue number | 5 | 19 | 5 |
| Protease cutting sites | |||
| Trypsin | 9 | 18 | 9 |
| Thermolysin | 32 | 28 | 20 |
| Pepsin (pH > 2.0) | 19 | 12 | 5 |
| Major sites of gene expression | Digestive glands | Labial palps, mantle | Digestive glands |
| Tissues with low mRNA level | Mantle, labial palps, gill, style sac-midgut, hemocytes | Gill, style sac-midgut, digestive gland, gonad | Style sac-midgut |