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Primers used in cv-lysozyme 3 cDNA cloning, and semi-quantitative RT-PCR
| Primer | Usage | Direction | Sequence |
|---|---|---|---|
| Lyso3-3' | 3'-RACE | - | 5'- CTT ACG GAA GCG ATG CAC CCT GCA CGA AC-3' |
| Lyso3-5' | 5'-RACE | - | 5'- CCG GTA GAA AAT GAG CCG GTG TGG GAG GC-3' |
| Lyso3-F | RT-PCR | Forward | 5'- GAG TTT AAA CTG AAC TTA AG-3' |
| Lyso3-R | RT-PCR | Reverse | 5'- CGT GAA CTT ATG TTT TAT TG-3' |
| 28S-F | RT-PCR | Forward | 5'- GTT GAC GCA ATG TGA TTT CTG C-3' |
| 28S-R | RT-PCR | Reverse | 5'-TAG ATG ACG AGG CAT TTG GCT A-3' |
