TABLE 1.

Details of African lyssavirus isolates used in this study and comparison of detection methods

Virus	Host	Country	Laboratory identification no.	GenBank accession no.	hnRT-PCRa		Real-time PCR
					JW primers	541lys and 550B	Cp	Copy no./reaction
Canid variant	Canine (Canis familiaris)	South Africa	262/06	Unpublished	+	+	21.51	1.17 × 106
Canid variant	Canine (C. familiaris)	South Africa	567/04	Unpublished	+	+	19.59	4.27 × 106
Canid variant	Canine (C. familiaris)	South Africa	479/96	Unpublished	+	+	20.69	2.04 × 106
Canid variant	Canine (C. familiaris)	South Africa	31/05	Unpublished	+	+	24.71	1.38 × 105
Mongoose variant	Mongoose (Galerella sanguinea)	Zimbabwe	22107	FJ392391	+	+	16.48	3.39 × 107
Mongoose variant	Mongoose (Cynictis penicillata)	South Africa	669/90	FJ392386	+	+	16.85	3.16 × 107
Mongoose variant	Mongoose (C. penicillata)	South Africa	767/95	FJ392388	+	+	16.48	2.69 × 107
Mongoose variant	Mongoose (C. penicillata)	South Africa	364/96	FJ392379	+	+	23.98	2.23 × 105
Mongoose variant	Mongoose (Atilax paludinosus)	South Africa	113/91	FJ392372	+	+	16.66	3.02 × 107
LBV	Bat (Epomophorus wahlbergi)	South Africa	LBV2008	Unpublished	+	+	23.34	3.47 × 105
LBV	Bat (Rousettus aegyptiacus)	Unknown	LBVAfr1999	EF547447	+	+	22.61	5.62 × 105
LBV	Bat (Eidolon helvum)	Nigeria	LBVNig1956	EF547459	−	+	31.49	1.48 × 103
LBV	Bat (E. wahlbergi)	South Africa	LBVSA2004	DQ 499945	+	+	19	6.17 × 106
LBV	Bat (A. paludinosus)	South Africa	LBVMong/2004	DQ 499948	+	+	20.46	2.37 × 106
MOKV	Feline (Felis domesticus)	Zimbabwe	12341	FJ465417	+	+	19	6.30 × 106
MOKV	Feline (F. domesticus)	South Africa	543/95	FJ465415	−	+	25.61	7.55 × 104
MOKV	Feline (F. domesticus)	South Africa	97/252	FJ465413	+	+	27.33	2.39 × 104
MOKV	Feline (F. domesticus)	South Africa	173/06	FJ465412	+	+	29.34	6.22 × 103
DUVV	Human	South Africa	DUVVSA/2006	DQ 676932	+	+	24.6	1.48 × 105
DUVV	Human	South Africa	DUVVSA/1971	EU623437	+	+	21.59	1.11 × 106

^a+, positive; −, negative.