Fig. 4. Keratin expression and basal lamina are altered in bi-transgenic mice. (A–D) Immunohistochemical staining of keratin-16 (A and B) and keratin-10 (C and D) on paraffin sections of K5-uPA/uPAR bi-transgenic mice (A and C) and K5-uPA transgenic mice (B and D). In bi-transgenic mice, the labelling for keratin-16 was increased (A), while that for keratin-10 (C) was decreased. (E and F) Indirect immunofluorescence of laminin-5 on cryosections of adult tail. The glycoprotein outlines the basal lamina in K5-uPA (F) and K5-uPA/uPAR mice (E). In the latter animals, at sites of epidermal detachment, laminin-5 distributes on both sides of the split (double-headed arrow). Bar = 100 µm.