TABLE 1.
Primers used in this study
| PCR | Reaction | Gene name | Primer | Primer sequence | Product size (bp) | Reference | Product (Tm [°C])a |
|---|---|---|---|---|---|---|---|
| Preliminary | 1 | narGHJI | LC66 | 5′AACCGACGGTGTGGTTGAC′3 | 155 | Stermann et al. (13) | |
| LC67 | 5′ATCTCGATGGATGGGCGTC′3 | ||||||
| 2 | oxyR | LC90 | 5′CGGGTGCCGCTGACCGCG′3 | 200 | Stermann et al. (13) | ||
| LC91 | 5′CCAGCCGGCTTCGCGTGG′3 | ||||||
| Real-time | 1 | narGHJI | narF | 5′CGCCGTCAACTTGGTTAGA′3 | M. tuberculosis (84.26 ± 0.09) | ||
| narR | 5′GTCCTGCCCGGAAGTTGT′3 | 108 | M. bovis (85.04 ± 0.02) | ||||
| 2 | oxyR | oxyF | 5′ACACTGATTCCGCAGACC′3 | M. bovis (91.9 ± 0.03) | |||
| oxyR | 5′AAAGTCAGCTCTGACAGCGC′3 | 151 | M. bovis (91.27 ± 0.04) |
a
The product melting temperatures (Tm) are given as means ± standard errors.