TABLE 1.

Sequences of primers and probes used in this study

Oligonucleotide	Sequence	Reference or source
CDC universal influenza A virus forward primer	5′-GAC CRA TCC TGT CAC CTC TGA C-3′	18
CDC universal influenza A virus reverse primer	5′-AGG GCA TTY TGG ACA AAK CGT CTA-3′
CDC universal influenza A virus probe	5′-(FAM)-TGC AGT CCT CGC TCA CTG GGC ACG-(BHQ1)-3′
CDC universal influenza B virus forward primer	5′-TCCTCAACTCACTCTTCGAGCG-3′	CDC
CDC universal influenza B virus reverse primer	5′-CGGTGCTCTTGACCAAATTGG-3′
CDC universal influenza B virus probe	5′-(JOE)-CCAATTCGAGCAGCTGAAACTGCGGTG-(BHQ1)-3′
H1N1 forward primer	5′-AAG CAA CAA AAA TGR AGG CAA TAC TA-3′	This study
H1N1 reverse primer	5′-TCT GTT GAA TTG TTC GCA TGA TAA-3′
H1N1 probe	5′-(FAM)-TTR CAA CCG CAA ATG CAG ACA CAT TAT G-(BHQ1)-3′
MS2 forward primer	5′-TGG CAC TAC CCC TCT CCG TAT TCA CG-3′	12
MS2 reverse primer	5′-GTA CGG GCG ACC CCA CGA TGA C-3′
MS2 probe	5′-(JOE)-CAC ATC GAT AGA TCA AGG TGC CTA CAA GC-(BHQ1a)-3′

^aBHQ1, Black Hole Quencher 1 dye.