FIG. 2.
(A) Diagram of the biochip for hybridization. Elements with the letter G contain genotype-specific probes. Four probes (G1-1 to G1-4) are used to identify genotype 1, three (G2-1 to G2-3) are used to identify genotype 2, two (G3-1 to G3-2) are used to identify genotype 3, three (G4-1 to G4-3) are used to identify genotype 4, three (G5-1 to G5-3) are used to identify genotype 5, and one (G6) is used to identify genotype 6. The probes for identifying subtypes are named ixN, where i is the genotype number, x indicates the subtype, and N is the number of the group corresponding to a segment of the NS5B region. (B) Fluorescence hybridization pattern of biochip elements obtained by analyzing an HCV sample of genotype 1, subtype 1b. The group of genotype-specific probes (G2-1 to G6) and groups 1 to 4 of subtype-specific probes of genotype 1 are outlined with a broken line. The histogram of normalized fluorescence signals of row 2 elements containing genotype-specific oligonucleotides is shown above the hybridization pattern. The histograms of normalized fluorescence signals of elements comprising groups 1 to 4 of subtype-specific oligonucleotides belonging to genotype 1 are outlined below the fluorescence image. The calculated value of the mean signal of empty elements (Iref) is shown by a solid bold line on all histograms.