Fig. 6. High expression of YIF1 rescues yip1 mutant cells. (A) Serial dilutions of yip1-2 mutant cells, transformed either with multicopy vector pRS326 or with the same vector containing the YIF1 or the YIP1 gene, were grown on agarose plates at permissive (25°C) or non-permissive conditions (37°C). (B) Invertase was induced and identified (as described in legend to Figure 4) in yip1-2 mutant cells transformed as in (A). Note that high expression of YIF1 significantly improves invertase glycosylation and secretion.