TABLE 4.

Comparison of sequencing and microarray for detection of amino acids at 16 selected positions^a

Position	No. of samples	Microarray identification		No. of samples identified by sequencing as:
				MUT																Ind
		Type	Amino acid	I	V	L	T	A	M	N	P	W	G	S	T	L	G	M	P		V	S	A	N	I	Y	R	D	H	C	*
rt169	164	WT	I	161													1
		MUT	T												0
			L													0	1
		Ind															1
rt173	164	WT	V		150												1														1
		MUT	L		3											8															1
			G														0
		Ind																													0
rt180	164	WT	L			104																									1
		MUT	M			2												28
			C																0
		Ind				14b												13c													2
rt181	164	WT	A				124																								1
		MUT	T														2
			G																	0
			V				2										12
		Ind					20d								2d		1d	0
rt184	164	WT	T				152																	1
		MUT	S				1														1										2
			A																			0
			G				1										0
			N																				0
			I																					1
		Ind					2																	3
rt194	164	WT	A					164
		MUT	T												0
		Ind																													0
rt202	163	WT	S											155
		MUT	I																					0
			G											5			0
		Ind												3e																	0
rt204	164	WT	M						104											1				2							3
		MUT	I						1															10
			V						2											21				12
			S																		0
		Ind							3f											3f				1f							0
rt236	123	WT	N							57
		MUT	T												2
			Y																						0
		Ind								58					6																0
rt250	114	WT	M						107
		MUT	V						5											0
		Ind							1g																						1h
s120	163	WT	P								53																				3
		MUT	T								1								0
			G														104
			A														2					0
		Ind																													0
s145	162	WT	G										56
		MUT	R										1													0
			A																			1
			T												1				10		2
			P																70							1
			S																1		13
		Ind																													0
preC28	29	WT	W									17																		2
		MUT	*									2																		8
			R																							0
		Ind																													0
preC29	22	WT	G										22
		MUT	D																								0
			H																									0
			S																		0
		Ind																													0
BCP1762	39	WT	A					14							2																1
		MUT	T					2							10
			G					2							4		0
		Ind						2							2																0
BCP1764	39	WT	G										14									1
		MUT	T										1		1							4							1
			A										2									8
			C										1									1							0
		Ind																				5									0

^aSamples with a reference Sanger sequence were tested with the microarray. The numbers of samples with a reference differ according to the codon and are indicated for each position. For each codon, either a wild type or one of the possible mutants (MUT) was identified by sequencing or the microarray. Ind, indeterminate; *, stop codon.

^bEleven arginines, 2 phenylalanines, 1 proline.

^cTwelve arginines, 1 lysine.

^dTwenty-three prolines.

^eOne valine, 1 cysteine and 1 glutamic acid.

^fSeven glycine.

^gAlanine.

^hIsoleucine by sequencing and threonine with the microarray.