TABLE 1.

Oligonucleotide primers used for MAC multiplex PCR

Genetic construct	Product size (bp)	Orientationa	Oligonucleotide sequence	Target organism(s)
IS900	398	F	5′ TGGACAATGACGGTTACGGAGGTGG 3′	M. avium subsp. paratuberculosis
		R	5′ CGCAGAGGCTGCAAGTCGTGG 3′
IS901	753	F	5′ GAACGCTGCTCTAAGGACCTGTTGG 3′	M. avium subsp. avium
		R	5′ GGAAGGGTGATTATCTGGCCTGC 3′
DT1	296	F	5′ CGTTGGCTGGCCATTCACGAAGGAGT 3′	M. avium subsp. avium and M. intracellulare
		R	5′ GCTAGTTGGATCGCGCCGAACACCGG 3′
IS1311	608	F	5′ GCGTGAGGCTCTGTGGTGAA 3′	All MAC members
		R	5′ ATGACGACCGCTTGGGAGAC 3′
16S rRNA gene	484	F	5′ ATAAGCCTGGGAAACTGGGT 3′	All mycobacterial species
		R	5′ CACGCTCACAGTTAAGCCGT 3′

^aF, forward; R, reverse.