TABLE 2.
Viral gene | Genome positiona | Oligonucleotide sense/function | 5′-3′ sense nucleotide sequence |
---|---|---|---|
H | 7315 | Forward primer | GAA CAC TCG GGT GGT TCT TAA TAA A |
7426 | Reverse primer | TGC GAT AGC TAA TAG CCC GAC | |
Probe | ACG GTG TTG TTT GTC ATG T | ||
F | 6956 | Forward primer | TGA TTG CAG TAG TGG GTA TCC TCA |
7092 | Reverse primer | TGA CCG TAC GTA GGA TTT GGA TG | |
Probe | ACC TGT TGC TGT AGG AAG | ||
N | 1020 | Forward primer | TGG GTG AAC TGG CTC C |
1120 | Reverse primer | CCC ATA GCA TAG CTC CA | |
Probe | TTC AGT GCA GGA GCA | ||
L9 | 9157 | Forward primer | GCAACATACAAACGGCTACCAA |
9303 | Reverse primer | TGGCAACCAGCTTGTTAGTCA | |
Probe | CTG TAT TTC ACC ATG GAC TC | ||
L10 | 10376 | Forward primer | RAT GAA AGG WCA TGC CAT ATT |
10450 | Reverse primer | GGT GGC CAG CTC C | |
Probe | ATC ATC AAC GGG TAT CG |
Numbers indicate the 5′ nucleotide position of the primer-probe, counting from the 5′ end of the full-length genome sequence of RPV, according to Kabete O sequence NC006296.