TABLE 7.
L10 rRT-PCR sensitivity on clinical samples from Kabete O RPV experimentally infected cattlea
| Sample and time point | Days p.i. | VI | rRT-PCR |
|---|---|---|---|
| In vivo | |||
| Nasal swab | 3 | 0/4 | 1/4 |
| 4 | 0/4 | 4/4 | |
| 5 | 4/4 | 4/4 | |
| Conjunctival swab | 3 | 0/4 | 2/4 |
| 4 | 3/4 | 3/4 | |
| 5 | 4/4 | 4/4 | |
| Oral swab | 3 | 0/4 | 0/4 |
| 4 | 0/4 | 2/4 | |
| 5 | 1/4 | 4/4 | |
| Rectal swab | 3 | 0/4 | 1/4 |
| 4 | 1/4 | 2/4 | |
| 5 | 2/4 | 3/4 | |
| Preputial swab | 3 | 1/2 | 2/2 |
| 4 | 2/2 | 2/2 | |
| 5 | 2/2 | 2/2 | |
| Plasma | 3 | 0/2 | 0/2 |
| 4 | 0/2 | 0/2 | |
| 5 | 0/2 | 0/2 | |
| WBC | 3 | 0/2 | 2/2 |
| 4 | 0/2 | 2/2 | |
| 5 | 1/2 | 2/2 | |
| Whole blood | 3 | 0/2 | 1/2 |
| 4 | 0/2 | 2/2 | |
| 5 | 0/2 | 2/2 | |
| Postmortem | |||
| Spleen | 6 | 6/6 | 6/6 |
| LN | 6 | 16/17 | 17/17 |
| Abomasum | 6 | 4/4 | 4/4 |
| Lingual tonsil | 6 | 2/2 | 2/2 |
| Palatine tonsil | 6 | 2/2 | 2/2 |
| Third eyelid | 6 | 2/2 | 2/2 |
| Peyer's patches | 6 | 3/3 | 3/3 |
| Gallbladder | 6 | 1/1 | 1/1 |
a
Results are expressed as the number of RPV rRT-PCR-positive animals out of the total number of infected animals tested. WBC, whole blood cells; LN, lymph node; p.i., postinfection; VI, virus isolation confirmed by conventional RT-PCR.