TABLE 2.

Primer sequences used to amplify rifampin and isoniazid resistance genes in M. tuberculosis

Primer namea	Sequence	Position	Length (no. of nucleotides)	% GC content	Tmb (°C)
rpoB gene
rpoB-F	5′-AGC CAG CTG AGC CAA TTC AT	2364	20	50.0	62.2
rpoB-R	5′-GCC CGG CAC GCT CAC GT	2472R	17	63.2	61.6
rpoB516-F	5′-AGG AGT TCT TCG GCA CCA G	2347	19	63.2	60.4
rpoB516-R	5′-GCA CGC TCA CGT GAC AGA C	2467R	19	70.6	61.8
katG gene
katG-F	5′-GGG CTG GAA GAG CTC GTA T	2872	19	57.9	53.1
katG-R	5′-CCG TAC AGG ATC TCG AGG AA	2991R	20	55.0	53.8
inhA promoter region
inhA-F	5′-CGT TAC GCT CGT GGA CAT AC	164	20	55.0	52.9
inhA-R	5′-TCC GGT AAC CAG GAC TGA AC	292R	20	55.0	53.6

^aThe GenBank accession numbers for the rpoB gene, katG gene, and inhA promoter region are L27989, X68081, and U66801, respectively.

^bT_m, melting temperature.