TABLE 5.

Results of high-resolution melting curve analysis for katG and inhA in M. tuberculosis isolates

Phenotype	No. of strains	katG		inhA
		Sequence analysis result	Type by HRM curve analysis	Sequence analysis result	Type by HRM curve analysis
INH-S	70	Wild	Wild	Wild	Wild
	47	NTc	Wild	NT	Wild
INH-R	10	Wild	Wild	Wild	Wild
	22	Wild	Wild	−15 C to Ta	Mutant
	1	D310A (GAC → GCC)	Variantb	−15 C to T	Mutant
	57	S315T (AGC → ACC)	Mutant	Wild	Wild
	10	S315T (AGC → ACC)	Mutant	−15 C to T	Mutant

^aNucleotide C-to-T substitution at position −15 in the promoter region of inhA.

^bThe melting curve of this type is clearly different from the curves for the wild and mutant types, so we defined it as a variant-type curve.

^cNT, not tested.