Phylogenetic tree based on the 5S-23S rRNA intergenic spacer region of different Borrelia species, constructed by neighbor-joining using Kimura 2-parameter and pairwise deletion with a bootstrap value of 500 replicates. Strains found in Östergötland County, Sweden, are shown in bold. Brackets denote Borrelia species clusters with more than 93% sequence similarity. The scale bar corresponds to the expected number of substitutions per nucleotide site. The reliability of the tree was tested by 500 bootstrap replicate analyses; only values greater than 50% are shown. The source of each reference sequence is indicated by an accession number preceded by a country code: CZ, Czech Republic; DE, Germany; FR, France; GB, Great Britain; MA, Morocco; SK, Slovakia; CH, Switzerland; TR, Turkey; RU, Russia.