Table I. Genotype analysis of newborn and E9.5 and E10.5 embryos from K18–/+K19–/– matings.
| Genotype | Newborn | E9.5 embryos | E10.5 embryos | |||
|---|---|---|---|---|---|---|
| |
Total No. |
Experimental ratio |
Total No. |
Experimental ratio |
Total No. |
Experimental ratio |
| K18–/+K19–/– | 257 | 2 | 74 | 2 | 33 | 1.9 |
| K18+/+K19–/– | 126 | 1 | 37 | 1 | 17 | 1 |
| K18–/–K19–/– | 0 | 0 | 32 | 0.9 | 13 | 0.8 |
| Average litter size | 6.5 | 9.6 | 10.3 | |||
The deviation from the mean seen with E10.5 K18–/–K19–/– embryos was due to a small number of embryos in the state of absorption. These could not be genotyped because of contamination with maternal tissue and blood. Adult animals were genotyped by PCR analysis. E9.5 and E10.5 embryos were dissected from the decidua and the extra-embryonic tissues and lysed for PCR analysis.