Table 1. Thermodynamic parameters for Ca_V1.2 IQ domain–Ca²⁺/CaM lobe interactions.

	WT–Ca2+/C lobe	WT–Ca2+/N lobe	F1628A–Ca2+/C lobe	F1628A–Ca2+/N lobe
N1	0.98 ± 0.18	0.85 ± 0.05	0.92 ± 0.04	0.96 ± 0.13
Kd1 (nM)	2.63 ± 0.07	57.6 ± 35.5	2.59 ± 0.24	1,003 ± 567
ΔH1 (kcal mol−1)	−6.77 ± 0.21	−1.91 ± 0.14	−3.69 ± 0.17	0.45 ± 0.02
ΔS1 (cal mol−1 K−1)	15.75 ± 0.78	26.70 ± 0.85	26.45 ± 0.78	29.20 ± 1.13
ΔG1 (kcal mol−1)	−11.31 ± 0.01	−9.60 ± 0.38	−11.31 ± 0.06	−7.96 ± 0.35
N2		1.04 ± 0.0		1.0 ± 0.0
Kd2 (nM)		19,190 ± 3,600		9,812 ± 8,220
ΔH2 (kcal mol−1)		−1.42 ± 0.13		−0.67 ± 0.22
ΔS2 (cal mol−1 K−1)		16.60 ± 0.84		21.00 ± 2.69
ΔG2 (kcal mol−1)		−6.20 ± 0.11		−6.71 ± 0.55

Thermodynamic parameters of Ca²⁺/C lobe and Ca²⁺/N lobe binding to wild-type (WT) and F1628A Ca_V1.2 IQ domain at pH 7.4 in the presence of 1 mM CaCl₂. Each value corresponds to the mean of two separate experiments with different batches of both components (± s.d.).