Table 1.
Primers used for the construction of expression vectors
Primer | Primer sequence | Restriction site |
Vector | Construct |
---|---|---|---|---|
OnbglF | 5′- CTTGCTTTATTCGCCAATTT -3′ | - | ||
OnbglR | 5′- TCTGCCGTTTCAAATAACGA -3′ | - | ||
Onbgl2F | 5′ - GATATACATATGTCTAAGATTACTTCAATTA -3′ | NdeI | pET-16b | pHM1 |
Onbgl2R | 5′- CGGATCCTCGAGTTAACTTTGATTGGCGAG -3′ | XhoI | ||
Onbgl3F | 5′ - CATATGTCTAAGATTACTTCAATTA - 3′ | NdeI | pET-21a | pHM2ATG |
Onbgl3R | 5′ - CTCGAGACTTTGATTGGCGAG - 3′ | XhoI | ||
TTGF | 5′ - AGATATACATTTGTCTAAGATTACTTC - 3′ | - | pET-21a | pHM2TTG |
TTGR | 5′ - ATGTATATCTCCTTCTTAAAGTTAAAC - 3′ | - | ||
Onbgl4F | 5′ - CATATGATGGTTTCCGATGGA - 3′ | NdeI | pET-21a | pHM3 |
LbbglF | 5′ - GATATACATATGGACATCGAACGAACGC - 3′ | NdeI | pET-21a | pHM4 |
LbbglR | 5′ - GTGGTGCTCGAGTTGACGTAATAAGGTGTTTGC - 3′ | XhoI |