Figure 6.

Inhibition of N-SMase, but not A-SMase, suppresses the activation of NF-κB in activated primary human astrocytes. A, Cells treated with 1 μm ASO or ScO against N-SMase and A-SMase for 40 h in complete media were stimulated with Aβ+IL-1β under the serum-free condition. After 1 h of stimulation nuclear extracts were prepared and subjected to EMSA for the detection of NF-κB. B, C, In the other set of experiments, cells preincubated with different concentrations of GW4869 (B) and imipramine (C) for 30′ were stimulated with Aβ+IL-1β for 1 h followed by EMSA. The top and bottom arrows indicate the induced NF-κB band and the unbound probe, respectively. Results represent three independent experiments.