FIGURE 5:

Small GTPase Rif enhances plasma membrane localization of mDia2 N-terminal constructs in a BD- and G-dependent manner. (A) Confocal microscopy images of HeLa cells coexpressing indicated GFP-mDia2 constructs (first row) and mRFP1 (second row) with (+Rif) or without (–Rif) Myc-Rif Q75L. Merged GFP/mRFP1 images are shown in the third row. Rif stained with Myc antibody is shown in white in the bottom row. Scale bar, 10 μm. GFP-G-DID localization is not changed by coexpression of active Rif, whereas localization of GFP-BD and, especially GFP-BD-G, is enhanced by Rif. (B) Average PM indices of indicated mDia2 constructs in the absence (blue) or presence (orange) of active Rif are shown with SEM. Statistically significant difference between PM indices of nonexpressing and Rif Q75L-expressing cells for a given GFP-mDia2 construct is marked by two asterisks for values of p < 0.01 and by one asterisk for values of p in the 0.01–0.05 range.