FIGURE 7:

Overexpression of anterograde ER-Golgi SNAREs and Sly1p partially rescues PH^WT inhibition. (A) Transport in wild-type (CBY3062) and overexpressor (CBY3061) semi-intact cells incubated for 20 min at 4°C with 3 μM PH^WT and/or 4 μM Sec18p followed by transport for 1 h at 23°C. (B) Microsomes treated as in panel A were mock treated (NA) or incubated with COPII proteins for 30 min at 23°C to monitor budding. (C) Indicated semi-intact cell acceptor membranes were pretreated with PH^WT and/or 4 μM Sec18p for 20 min at 4°C, then were incubated with COPII vesicles containing [³⁵S]gpαf in the presence of fusion factors for 1 h at 23°C. The –Acpt condition indicates background fusion in the absence of added acceptor membranes. The data in panel C are plotted relative to control transport levels in Supplemental Figure S4.