Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Jan 15;22(2):266–277. doi: 10.1091/mbc.E10-07-0605

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 Ishitani et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,“ “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 2: — Phosphorylation of NLK is required for kinase activity. (A) Flowchart for the hyperautophosphorylation and dephosphorylation of NLK. (B) Untreated Flag-NLK, hyperautophosphorylated Flag-NLK, and dephosphorylated Flag-NLK were prepared as described in Materials and Methods. Flag-Tcf4 was expressed in HEK293 cells, and the proteins were immunopurified. Aliquots of purified Tcf4 and NLK proteins were subjected to non-RI in vitro kinase assay for 15 or 30 min and then immunoblotted with anti-Flag antibody. Purified NLK proteins were confirmed by immunoblotting with anti-Flag antibody (bottom).