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. 2011 Jan 15;22(2):266–277. doi: 10.1091/mbc.E10-07-0605

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© 2011 Ishitani et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — NLK forms dimers. Size-exclusion chromatography was performed with lysates of HEK293 cells expressing Flag-NLK (WT), NLK(K155M) (KM), NLK(T286V) (TV), NLK(C425Y) (CY), or NLK(1–415/K155M) (N-KD) on Superdex 200 column. Next, 0.25-ml fractions were collected, and aliquots from each fraction were electrophoresed through SDS–PAGE and immunoblotted with anti-Flag antibody. The predicted sizes of NLK dimers and monomers and the molecular-weight size markers are indicated at the top of the panel.