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. 2011 Jan 13;7(1):e1001256. doi: 10.1371/journal.ppat.1001256

Figure 5. Endothelial cells retain EC-tropic virus particles.

Figure 5

HFF were infected with vBAC4-luc as described in Materials and Methods to obtain equal numbers of initially infected cells (m.o.i. on HFF: 0.2). Six days after infection supernatants were harvested, cells homogenized as described in Materials and Methods and different fractions of the homogenates tested on HFF and TIME cells for their infection capacities by luciferase assay. (A) shows the TIME/HFF infection ratios and (B) the absolute luciferase activities of the different cell preparations on HFF and TIME cells. Shown are means +/− SD of three independent experiments assayed in triplicates. For HFF all homogenate preparations were significantly less EC-tropic than the cell culture supernatants. For HUVEC the total homogenates and the resuspended pellets were significantly more EC-tropic than the cell culture supernatants (Student's t test).