Fig. 3. Overexpression of MOF¹ in S2 cells causes a reduction in the level of H4Ac16 present on the X chromosomes and relocation of MSL complexes. Histone H4Ac16 in S2 cells transfected with the vector (A) or with mof¹ cDNA (B) and induced with CuSO₄. (C) Merged image of induced S2-mof¹ cells stained for MSL1 (green) and counterstained for nucleic acids with propidium iodide (red). The yellow color indicates the presence of the MSL1 protein.