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. 2011 Jan 13;6(1):e16078. doi: 10.1371/journal.pone.0016078

Figure 2. Noggin silencing has no effect on cell proliferation and affects only minimally the expression of osteotropic factors, in vitro.

Figure 2

A. In vitro proliferation of mock and Nog-KD clones was measured by BrdU incorporation for 4 days and compared to PC-3/Fluc cells. Average values of 3 independent experiments performed in quadruplicate wells (+/− SD). B. Expression of Dkk-1, PTHrP, RANKL, CSF-1 and IL-8 mRNA. mRNA expression levels (+/− SD) are quantified by real-time RT-PCR and normalized to β-actin as endogenous control. The mRNA expression level in PC-3/Fluc cells is set as 100%; the mean of 2 to 3 independent experiments is shown. ***P<0.001, mock and Nog-KD 17 clones versus PC-3/Fluc and Nog-KD 14 clones.